Web Release Date: March 16,
Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization in Transmission Geometry
and
Department of Chemistry, The George Washington University, Washington, D.C. 20052, and Chemical Dynamics and Diagnostics Branch, Naval Research Laboratory, Code 6115, Washington, D.C. 20375
Received for review October 17, 2001. Accepted December 13, 2001.
Abstract:
In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, the laser typically illuminates the analyte on the front side of an opaque surface (reflection geometry). Another configuration consisting of laser illumination through the sample backside (transmission geometry) has been used in conventional MALDI; however, its use and the number of reports in the literature are limited. The viability of transmission geometry with AP MALDI is demonstrated here. Such a geometry is simple to implement, eliminates the restriction for a metallic sample holder, and allows for the potential analysis of samples on their native transparent surfaces, e.g., cells or tissue sections on slides.
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