Web Release Date: February 18,
SPR Imaging of Photo-Cross-Linked Small-Molecule Arrays on Gold
and
Antibiotics Laboratory and Beam Application Team, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, and Biotechnology Frontier Project, TOYOBO Co. Ltd., 10-24 Toyo-cho, Tsuruga, Fukui 914-0047, Japan
Received for review October 5, 2005. Accepted January 21, 2006.
Abstract:
Identification of small-molecule ligands for a protein of
interest can facilitate the analysis of the protein's functions
in biological systems. Small-molecule microarrays have
allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on
a protein is needed to observe these interactions. By
combining SPR imaging technology with our recently
developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ
observation of interactions between photo-cross-linked
small molecules on gold surfaces and nonlabeled proteins
in solution. Interactions of estrogenic and androgenic
substances with estrogen receptor 
were observed using
this platform.
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