Web Release Date: June 22,
Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation
Laboratoire d'Electrochimie Physique et Analytique, Station 6, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland
Received for review March 9, 2007. Accepted May 22, 2007.
Abstract:
Capillary zone electrophoresis and carrier ampholytes
based capillary electrophoresis have been used as a
second separation step to Off-Gel isoelectric focusing for
the analysis of complex peptide mixtures. A tryptic digest
of four proteins (bovine serum albumin, 
-lactoglobulin,
horse myoglobin, cytochrome c) has been chosen as a
peptide test mixture. After assessment of different modes
of capillary electrophoresis as a second dimension to
Off-Gel isoelectric focusing, the optimized two-dimensional platforms provide a degree of orthogonality comparable to state-of-the-art multidimensional liquid chromatography systems as well as a practical peak capacity
above 700.
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