Web Release Date: February 21,
Gold Nanohole Array Substrates as Immunobiosensors
and
The Horticulture and Food Research Institute of New Zealand, East Street, Private Bag 3123, Hamilton 3240, New Zealand, MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Private Bag 4800, Christchurch 8020, New Zealand, and Department of Engineering, University of Waikato, Private Bag 3015, Hamilton 3240, New Zealand
Received for review December 17, 2007. Accepted January 21, 2008.
Abstract:
A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.
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