Web Release Date: January 25,
Alignment-Based Approach for Durable Data Storage into Living Organisms
and
Institute for Advanced Biosciences, Keio University, 403-1 Nipponkoku, Daihoji, Tsuruoka, 997-0017 Yamagata, Japan; Bioinformatics Program, Graduate School of Media and Governance, Keio University, 5322 Endo, Fujisawa, 252-8520 Kanagawa, Japan; Department of Environmental Information, Keio University, 5322 Endo, Fujisawa, 252-8520 Kanagawa, Japan; and Human Metabolome Technologies, Inc., 246-2 Mizukami, Kakuganji, Tsuruoka, 997-0052 Yamagata, Japan
Received August 28, 2006. Accepted December 18, 2006.
Abstract:
The practical realization of DNA data storage is a major scientific goal. Here we introduce a simple, flexible, and robust data storage and retrieval method based on sequence alignment of the genomic DNA of living organisms. Duplicated data encoded by different oligonucleotide sequences was inserted redundantly into multiple loci of the Bacillus subtilis genome. Multiple alignment of the bit data sequences decoded by B. subtilis genome sequences enabled the retrieval of stable and compact data without the need for template DNA, parity checks, or error-correcting algorithms. Combined with the computational simulation of data retrieval from mutated message DNA, a practical use of this alignment-based method is discussed.
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