Web Release Date: April 23,
Enzymic Thin Film Coatings for Bioactive Materials
and
Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108, Department of Chemical and Biomolecular Engineering, University of Akron, Akron, Ohio 44325, and Materials Research Department, Toyota Motor Engineering and Manufacturing North America, Inc., Ann Arbor, Michigan 48105
Received October 30, 2007. Accepted March 24, 2008.
Abstract:
Bioactive materials have been explored for a broad range of applications including biocatalysts,
biosensors, antifouling membranes and other functional and smart materials. We report herein
a unique method for preparation of bioactive materials through a spin coating process. Specifically,
we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined
their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic
coatings with a typical loading of 13 
g/cm2, retaining 46% of the enzyme activity for hydrolysis
of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed
the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA
that was coated on top of the enzyme thin film.
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