Web Release Date: October 24,
Intraparticle Energy Transfer and Fluorescence Photoconversion in Nanoparticles: An Optical Highlighter Nanoprobe for Two-Photon Bioimaging
and
Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, State University of New York, Buffalo, New York 14260-3000, and Advanced Photonics Center, Southeast University, Nanjing, China 210096
Received May 10, 2007
Abstract:
We report organically modified silica nanoparticles co-encapsulating two-photon fluorescent dye aggregates and a red-fluorescent energy acceptor, where the latter is indirectly excited through intraparticle energy transfer following two-photon excitation of the former. In vitro two-photon microscopy has demonstrated that these nanoprobes are avidly internalized into cells with bright acceptor fluorescence and that cellular optical highlighting is effected with red-to-green photoconversion by selective acceptor bleaching, while keeping the donor and the nanostructure of particles intact.
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