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Communication

Chemically Induced Hairpin Formation in DNA Monolayers

Supporting Info

Emily A. Smith,^† Motoki Kyo,^† Hiroyuki Kumasawa,^‡ Kazuhiko Nakatani,^‡^§ Isao Saito,^‡ and Robert M. Corn*^†

Department of Chemistry, University of Wisconsin, Madison Wisconsin 53706, and Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and PREST, Japan Science and Technology Corporation, Kyoto 606-8501, Japan

J. Am. Chem. Soc., 2002, 124 (24), pp 6810–6811

DOI: 10.1021/ja026356n

Publication Date (Web): May 21, 2002

†

University of Wisconsin.

‖

Current address: Toyobo Co. Ltd. Bio 21 Project, 10-24 Toyo-cho Tsuruga Fukui 914-0047, Japan.

‡

Kyoto University.

PREST, Japan Science and Technology Corporation.

To whom correspondence should be addressed. E-mail: corn@chem.wisc.edu.

Abstract

A naphthyridine dimer that binds specifically to G−G mismatches has been used to induce hairpin formation in oligonucleotides immobilized onto chemically modified gold surfaces. Surface plasmon resonance (SPR) imaging measurements of DNA microarrays were used to demonstrate that binding of the naphthyridine dimer to G−G mismatches within the stem portion of an immobilized 42-mer oligonucleotide could be used to induce hairpin formation that prevented hybridization of DNA complementary to the loop sequence. In addition, the selectivity of the naphthyridine dimer for G−G mismatches was verified through SPR imaging measurements of the hybridization adsorption of an 11-mer oligonucleotide to a four-component DNA array of zero- and single-base mismatch sequences.

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