J. Am. Chem. Soc., 125 (22), 6612 -6613, 2003. 10.1021/ja028200t S0002-7863(02)08200-8
Web Release Date: May 10, 2003

Copyright © 2003 American Chemical Society

Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation

Nathan Lamarre-Vincent and Linda C. Hsieh-Wilson*

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125

lhw@caltech.edu

Received August 19, 2002

Abstract:

We report that CREB (cyclic AMP-responsive element-binding protein), a transcription factor essential for long-term memory, is O-GlcNAc glycosylated in the mammalian brain. Glycosylation occurs at two sites within the Q2 domain and disrupts the interaction between CREB and TAFII130, thereby repressing the transcriptional activity of CREB in vitro. These findings have important implications for the role of O-GlcNAc glycosylation in gene regulation, and they provide a link between O-GlcNAc and information storage processes in the brain.

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