Prev. Article Next Article Table of Contents

Communication

RNA Guanine Quadruplex Invasion by Complementary and Homologous PNA Probes

Supporting Info

Violeta L. Marin and Bruce A. Armitage*

Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213

J. Am. Chem. Soc., 2005, 127 (22), pp 8032–8033

DOI: 10.1021/ja051102y

Publication Date (Web): May 14, 2005

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

army@andrew.cmu.edu

Abstract

Guanine quadruplexes are gaining increasing attention due to their suspected roles in regulating gene expression at the transcriptional and translational levels. This paper describes the ability of short peptide nucleic acid (PNA) probes to disrupt a stable RNA quadruplex and hybridize to their target sequence. In one case, the PNA probe is complementary to the target, resulting in formation of a Watson−Crick base-paired duplex. In the second case, the PNA probe is homologous to the target and forms a hybrid quadruplex structure. The hybrid duplex is formed in a 1:1 stoichiometry, as expected based on the constraints imposed by Watson−Crick pairing. However, the hybrid quadruplex is formed in a PNA₂:RNA stoichiometry, due to the ability of the short PNA to hybridize with both halves of the original RNA quadruplex.

View: Full Text HTML | Hi-Res PDF