Building Addressable Libraries: The Use of a Mass Spectrometry Cleavable Linker for Monitoring Reactions on a Microelectrode Array

J. Am. Chem. Soc., 128 (50), 16020 -16021, 2006. 10.1021/ja067194o S0002-7863(06)07194-0
Web Release Date: November 24, 2006

Building Addressable Libraries: The Use of a Mass Spectrometry Cleavable Linker for Monitoring Reactions on a Microelectrode Array

Ceng Chen, Gabriella Nagy, Amy V. Walker,* Karl Maurer, Andy McShea, and Kevin D. Moeller*

Department of Chemistry, Washington University, St. Louis, Missouri 63130, and CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, Washington 98275

moeller@wuchem.wustl.edu

Received October 7, 2006

Abstract:

Time-of-flight secondary ion mass spectrometry (TOF SIMS) has been used in conjunction with a mass spectrometry cleavable linker to determine the percent conversion of reactions that were conducted site-selectively on an addressable microelectrode array. When combined with fluorescence techniques for analysis of the reactions, the TOF SIMS experiment provides a means for optimization of both reaction confinement and reaction efficiency on the microelectrode arrays.

Download the full text: PDF | HTML