Web Release Date: May 25,
Single-Molecule Conformational Analysis of G-Quadruplex Formation in the Promoter DNA Duplex of the Proto-Oncogene C-Kit
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Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom, Department of Physics and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Illinois 61801, and Biological Nanoscience Section, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, United Kingdom
Received January 23, 2007
Abstract:
Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.
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