Web Release Date: January 29,
Shape-Control of Protein Crystals in Patterned Microwells
and
Department of Chemistry and Department of Materials Science and Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113
Received October 16, 2007
Abstract:
This Communication demonstrates how selectively functionalized, patterned microscale wells can be used as small-volume (fL) crystallization vessels to grow protein crystals. This surface-patterned approach enables proteins to be crystallized with well-controlled shapes and uniform sizes from 3 
m down to 600 nm. Three different proteins-lysozyme, thaumatin, and glucose isomerase-were crystallized as model systems to demonstrate the generality of this technique. Different polymorphs were formed simply by controlling the evaporation conditions.
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