J. Agric. Food Chem., 44 (3), 701 -705, 1996. jf950579y

Copyright © 1996 American Chemical Society

Total Antioxidant Capacity of Fruits

Hong Wang, Guohua Cao, and Ronald L. Prior*

Jean Mayer Human Nutrition Research Center on Aging at Tufts University, Agricultural Research Service, U.S. Department of Agriculture, Boston, Massachusetts 02111, and Nutritional Science Department, University of Connecticut, Storrs, Connecticut 06296

Received for review August 23, 1995. Revised manuscript received December 4, 1995. Accepted December 18, 1995.

Abstract:

The total antioxidant activity of 12 fruits and 5 commercial fruit juices was measured in this study using automated oxygen radical absorbance capacity (ORAC) assay. On the basis of the wet weight of the fruits (edible portion), strawberry had the highest ORAC activity (micromoles of Trolox equivalents per gram) followed by plum, orange, red grape, kiwi fruit, pink grapefruit, white grape, banana, apple, tomato, pear, and honeydew melon. On the basis of the dry weight of the fruits, strawberry again had the highest ORAC activity followed by plum, orange, pink grapefruit, tomato, kiwi fruit, red grape, white grape, apple, honeydew melon, pear, and banana. Most of the antioxidant capacity of these fruits was from the juice fractions. The contribution of the fruit pulp fraction (extracted with acetone) to the total ORAC activity of a fruit was usually less than 10%. Among the commercial fruit juices, grape juice had the highest ORAC activity followed by grapefruit juice, tomato juice, orange juice, and apple juice.

Keywords: Free radicals; antioxidant; peroxyl radical; fruits

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