J. Agric. Food Chem., 50 (11), 3112 -3115, 2002. 10.1021/jf0116101 S0021-8561(01)01610-7
Web Release Date: April 30, 2002

Copyright © 2002 American Chemical Society

Determination of Antioxidant Power of Red and White Wines by a New Electrochemical Method and Its Correlation with Polyphenolic Content

Ángeles M. Alonso,* Cristina Domínguez, Dominico A. Guillén, and Carmelo G. Barroso

Departamento de Química Analítica, Facultad de Ciencias, Universidad de Cádiz, C/República Saharaui, s/n. 11510 Puerto Real, Cádiz, Spain

Received for review December 7, 2001. Revised manuscript received February 28, 2002. Accepted March 4, 2002. This study was supported by the CICYT of the Spanish Ministry of Education and Science and FEDER of U.E. (Project 1FD97-0683-C05-01).

Abstract:

A new method for measuring the antioxidant power of wine has been developed based on the accelerated electrochemical oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). The calibration (R = 0.9922) and repeatability study (RSD = 7%) have provided good statistical parameters. The method is easy and quick to apply and gives reliable results, requiring only the monitoring of time and absorbance. It has been applied to various red and white wines of different origins. The results have been compared with those obtained by the total antioxidant status (TAS) method. Both methods reveal that the more antioxidant wines are those with higher polyphenolic content. From the HPLC study of the polyphenolic content of the same samples, it is confirmed that there is a positive correlation between the resveratrol content of a wine and its antioxidant power.

Keywords: Antioxidant activity; wine; ABTS; polyphenols; resveratrol


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