Web Release Date: June 25,
Evaluation of Antioxidant Capacity of Cereal Brans




and

Department of Food Science, Veterinary Faculty, Campus de Espinardo, University of Murcia, Apartado de Correos 4021, E-30008 Murcia, Spain, Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy, Istituto di Biotecnologie Biochimiche, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy, and I+D Department, Casa Santiveri S.A., 08038 Barcelona, Spain
Received for review March 8, 2004. Revised manuscript received May 11, 2004. Accepted May 17, 2004. This work was supported by an University of Murcia project (Ref. UMU-5142).
Abstract:
Several oat brans (crunchy oat bran, oat bran alone, and oat breakfast cereal) and wheat brans
(wheat bran alone, wheat bran powder, wheat bran with malt flavor, bran breakfast cereal, tablet of
bran, and tablet of bran with cellulose) used as dietary fiber supplements by consumers were evaluated
as alternative antioxidant sources (i) in the normal human consumer, preventing disease and promoting
health, and (ii) in food processing, preserving oxidative alterations. Products containing wheat bran
exhibited higher peroxyl radical scavenging effectiveness than those with oat bran. Wheat bran powder
was the best hydroxyl radical (OH
) scavenger. In terms of hydrogen peroxide (H2O2) scavenging,
wheat bran alone was the most effective, while crunchy oat bran, oat bran alone, and oat breakfast
cereal did not scavenge H2O2. The shelf life of fats (obtained by the Rancimat method for butter)
increased most in the presence of crunchy oat bran. When the antioxidant activity during 28 days of
storage was measured by the linoleic acid assay, all of the oat and wheat bran samples analyzed
showed very good antioxidant activities. The Trolox equivalent antioxidant capacity (TEAC) assay
was used to provide a ranking order of antioxidant activity. The wheat bran results for TEAC (6 min),
in decreasing order, were wheat bran powder > wheat bran with malt flavor
wheat bran alone
bran breakfast cereal > tablet of bran > tablet of bran with cellulose. The products made with oat
bran showed lower TEAC values. In general, avenanthramide showed a higher antioxidant level than
each of the following typical cereal components: ferulic acid, gentisic acid, p-hydroxybenzoic acid,
protocatechuic acid, syringic acid, vanillic acid, vanillin, and phytic acid.
Keywords: Antioxidant; wheat; oat; bran
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