Web Release Date: September 25,
Effective Separation of Potent Antiproliferation and Antiadhesion Components from Wild Blueberry (Vaccinium angustifolium Ait.) Fruits
Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, 1201 South Dorner Drive, Urbana, Illinois 61801; Phillip E. Marucci Center for Blueberry and Cranberry Research, Rutgers University, 125A Lake Oswego Road, Chatsworth, New Jersey, 08019; Department of Plant Biology, University of Illinois at Urbana-Champaign, 505 South Goodwin Avenue, Urbana, Illinois 61801; and Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 905 South Goodwin Avenue, Urbana, Illinois 61801
Received for review May 12, 2004. Revised manuscript received August 16, 2004. Accepted August 16, 2004. We thank the University of Illinois Department of Natural Resources and Environmental Sciences and the Michigan State Horticultural Society for scholarship funding. This research project was funded in part by NIH/NCCAM R21 Grant AT000404-01 and USDA/IFAS Grant 00-52101-9695. The Quattro mass spectrometer was purchased in part with a grant from the Division of Research Resources, National Institutes of Health (RR 07141). The Voyager-DE STR mass spectrometer was purchased in part with a grant from the Division of Research Resources, National Institutes of Health (RR 11966).
Abstract:
Extracts from wild blueberry (Vaccinium angustifolium Ait.) were separated into proanthocyanidin-rich fractions using liquid vacuum and open column chromatography on Toyopearl and Sephadex
LH-20, respectively. Fractions were characterized using analytical tools including mass spectrometry
and NMR spectroscopy; fraction composition was correlated with bioactivity using antiproliferation
and antiadhesion in vitro assays. There was a significant positive correlation between proanthocyanidin
content of different fractions and biological activity in both the antiproliferation and antiadhesion assays.
Two fractions containing primarily 4
8-linked oligomeric proanthocyanidins with average degrees of
polymerization (DPn) of 3.25 and 5.65 inhibited adhesion of Escherichia coli responsible for urinary
tract infections. Only the fraction with a DPn of 5.65 had significant antiproliferation activity against
human prostate and mouse liver cancer cell lines. These findings suggest both antiadhesion and
antiproliferation activity are associated with high molecular weight proanthocyanidin oligomers found
in wild blueberry fruits.
Keywords: Vaccinium angustifolium Ait.; blueberry; antiproliferation; antiadhesion; proanthocyanidin; condensed tannins
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