Web Release Date: October 26,
The Development of a Semiautomated Procedure for the Synthesis and Screening of a Large Group of Molecularly Imprinted Polymers
Department of Chemistry, Oregon State University, Corvallis, Oregon, Johnson and Johnson Pharmaceutical Research and Development, LLC, P.O. Box 300, Route 202, Raritan, New Jersey, and Department of Biomedical Engineering, Rutgers University, New Brunswick, New Jersey
Received February 13, 2007
Abstract:
A method for synthesis and evaluation of molecularly imprinted polymers (MIPs) on a semiautomated miniature scale is reported. This technique combines molecular imprinting with the combinatorial chemistry approach, allowing rapid screening and optimizations of libraries of MIPs. The polymers were prepared and evaluated in situ by rebinding utilizing powder dispensing and liquid handling systems. MIPs were prepared by a combinatorial approach using methacrylic acid (MAA), 4-vinylpyridine (4-VP), acrylamide, and styrene as functional monomers, and acetonitrile and toluene as porogenic solvents. A drug substance having aromatic, hydroxyl, –O–CONH2 functional groups was selected as the template molecule for this study. The MIP library results demonstrated that the polymer prepared with MAA as functional monomer shows the strongest binding affinity, and therefore, is preferred for the preparation of this particular template molecule. Due to the low consumption of reagents, and more importantly, the demonstrated ability of this method to effectively identify optimal imprinting conditions, this small-scale combinatorial protocol is well suited for fast and efficient screening and optimizations of MIPs.
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