Phillip W. Snyder,Matthew S. Johannes,Briana N. Vogen,Robert L. Clark,* andEric J. Toone*
Department of Chemistry, Duke University, Durham, North
Carolina, 27708-0346, and The Pratt School of Engineering,
Duke University, Durham, North Carolina, 27708-0271
rclark@egr.duke.edu; eric.toone@duke.edu
Received June 7, 2007
Abstract:
Immobilized biocatalytic lithography is presented as an
application of soft lithography. In traditional microcontact
printing, diffusion limits resolution of pattern transfer. By
using an immobilized catalyst, the lateral resolution of
microcontact printing would depend only on the length and
flexibility of the tether (<2 nm) as opposed to diffusion
(>100 nm). In the work, exonuclease reversibly immobilized
on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal
fluorescence microscopy to be ~70%.
