Web Release Date: February 8,
Fluorescent II-VI Semiconductor Quantum Dots in Living Cells: Nonlinear Microspectroscopy in an Optical Tweezers System
and
Departamento de Biofísica e Radiobiologia, UFPE, Recife, PE, Brazil, Departamento de Ciências Farmacêuticas, UFPE, Recife, PE, Brazil, Instituto de Física Gleb Wataghin, UNICAMP, Campinas, SP, Brazil, and Departamento de Química Fundamental, UFPE, Recife, PE, Brazil
Received: July 25, 2007
In Final Form: December 12, 2007
Abstract:
In this work we used a setup consisting of an optical tweezers combined with a nonlinear microspectroscopy system to perform scanning microscopy and obtain emission spectra using two photon excited (TPE) luminescence of captured single living cells labeled with core-shell fluorescent semiconductor quantum dots (QDs). The QDs were obtained via colloidal synthesis in aqueous medium with an adequate physiological resulting pH. Sodium polyphosphate was used as the stabilizing agent. The results obtained show the potential presented by this system as well as by these II-VI fluorescent semiconductor quantum dots to perform spectroscopy in living trapped cells in any neighborhood and dynamically observe the cell chemical reactions in real time.
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