Proteomic Analysis of Cervical-Vaginal Fluid: Identification of Novel Biomarkers for Detection of Intra-amniotic Infection

J. Proteome Res., 6 (1), 89 -96, 2007. 10.1021/pr060149v S1535-3893(06)00149-7
Web Release Date: November 23, 2006

Proteomic Analysis of Cervical-Vaginal Fluid: Identification of Novel Biomarkers for Detection of Intra-amniotic Infection

Michael G. Gravett,* Archana Thomas, Kimberly A. Schneider, Ashok P. Reddy, Surendra Dasari, Thomas Jacob, Xinfang Lu, Matthew Rodland, Leonardo Pereira, Drew W. Sadowsky, Charles T. Roberts, Jr., Miles J. Novy, and Srinivasa R. Nagalla

Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, Oregon 97006, Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195, Departments of Obstetrics & Gynecology and Pediatrics, Oregon Health and Science University, Portland, Oregon 97239, and ProteoGenix, Inc., Portland, Oregon 97213

Received April 6, 2006

Abstract:

Intra-amniotic infection (IAI) is associated with preterm birth and perinatal mortality. To identify potential biomarkers, we performed a comprehensive survey of the cervical-vaginal fluid (CVF) proteome from a primate IAI model utilizing multidimensional protein identification technology (LC/LC-MS/MS) and MALDI-TOF-MS analyses. Analyses of CVF proteome identified 205 unique proteins and differential expression of 27 proteins in controls and IAI samples. Protein expression signatures and immunodetection of specific biomarkers identified can be employed for noninvasive detection of IAI.

Keywords: intra-amniotic infection preterm birth cervical-vaginal fluid

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