Web Release Date: June 24,
Microbicides for HIV/AIDS. 1. Electrophoretic
Fingerprinting the H9 Cell Model System
and
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, International Partnership for Microbicides, Silver Spring, Maryland 20910, St. George's Hospital Medical School, London SW17 0RE, United Kingdom, and Malvern Instruments Inc., Southborough, Massachusetts 01772
Received March 7, 2005
In Final Form: May 7, 2005
Abstract:
An electrophoretic fingerprint of a CD4+ T-cell (H9) has been produced for the first time. Samples were taken from three separate cultures prepared at different times to obtain a general characterization of the cells. The availability of commercial instrumentation equipped with an auto-titrator has made possible the application of both the 2-dimensional and 3-dimensional representation of electrophoretic fingerprinting. The 2-dimensional treatment has been used to assess the reliability of the data and has detected hysteresis as a possible second-order effect. The 3-dimensional representation has been used to explore the data needed for a reliable overall pattern that characterizes the conditions of pH and conductivity required for an effective microbicide. The dome negative maximum in the electrophoretic fingerprint at high pH, along with the line of zero mobility (LZM) and a dome positive maximum at low pH, are interpreted as evidence for surface carboxyl groups prominent in the alkaline regime and surface amino groups prominent in the acid regime, suggesting that the H9 cell surface is zwitterionic. This has important implications as to the choice and design of microbicide actives.
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