Web Release Date: November 22,
Structure and Orientation Changes of 
- and 
-Gliadins at the
Air-Water Interface: A PM-IRRAS Spectroscopy and Brewster
Angle Microscopy Study
and
Centre de Recherche Paul Pascal, UPR 8641-CNRS, Avenue Albert Schweitzer, F-33600 Pessac, Laboratoire de Chimie et Biologie des Membranes et Nanoobjets, UMR 5248-CNRS, Université Bordeaux 1, ENITAB, 2 rue Robert Escarpit, F-33607 Pessac, and UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes
Received July 9, 2007
In Final Form: September 22, 2007
Abstract:
Microscopic and molecular structures of - and -gliadin monolayers at the air-water interface were studied under
compression by three complementary techniques: compression isotherms, polarization modulation infrared reflection
absorption spectroscopy (PM-IRRAS), and Brewster angle microscopy (BAM). For high molecular areas, gliadin
films are homogeneous, and a flat orientation of secondary structures relative to the interface is observed. With
increasing compression, the nature and orientation of secondary structures changed to minimize the interfacial area.
The -gliadin film is the most stable at the air-water interface; its interfacial volume is constant with increasing
compression, contrary to -gliadin films whose molecules are forced out of the interface. -Gliadin stability at a high
level of compression is interpreted by a stacking model.
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