Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates

Nano Letters, 1 (1), 32 -35, 2001. 10.1021/nl005508e S1530-6984(00)05508-9
Web Release Date: December 16, 2000

Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates

Daniela Zanchet, Christine M. Micheel, Wolfgang J. Parak, Daniele Gerion, and A. Paul Alivisatos*

Department of Chemistry, University of California, Berkeley, and Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley California 94720-1460

Received October 5, 2000

Abstract:

Colloidal nanocrystal/DNA conjugates hold the promise of becoming powerful probes for biological diagnostics as well as versatile building blocks for nanotechnology. To fully realize this potential, it is important to precisely control the number of oligonucleotides bound to the nanocrystal. Here we demonstrate electrophoretic isolation of 5 and 10 nm gold nanocrystals bearing discrete numbers of single-stranded DNA (1-5). The potential use of these discrete conjugates in the fabrication of novel nanostructures is discussed.

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