Web Release Date: September 29,
Calibration of Dynamic Molecular Rulers Based on Plasmon Coupling between Gold Nanoparticles
and
Physics Department, Biophysics Graduate Program, and Chemistry Department, University of California, Berkeley, California 94720, and Physical Biosciences Division and Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720
Received August 12, 2005
Revised September 9, 2005
Abstract:
Pairs of noble metal nanoparticles can be used to measure distances via the distance dependence of their plasmon coupling. These "plasmon rulers" offer exceptional photostability and brightness; however, the advantages and limitations of this approach remain to be explored. Here we report detailed plasmon peak versus separation calibration curves for 42- and 87-nm-diameter particle pairs, determine their measurement errors, and describe experimental procedures to improve their performance in biology, nanotechnology, and materials sciences.
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