Web Release Date: June 29,
Identity Profiling of Cell Surface Markers by Multiplex Gold Nanorod Probes
# and
Department of Agricultural and Biological Engineering and Bindley Biosciences Center, Purdue University, 225 South University Drive, West Lafayette, Indiana 47906, Department of Surgery, Walther Oncology Center, Department Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, Walther Cancer Institute, Indianapolis, Indiana 46208
Received April 16, 2007
Revised May 18, 2007
Abstract:
Gold nanorod molecular probes (GNrMPs) were designed and fabricated for multiplex identification of cell surface markers in HBECs. Cells were probed directly using dark field microscopy integrated with a spectral imager for simultaneous detection of up to three surface markers. The immunophenotype composition of these cell lines indicative of their metastasis potential was assessed using the GNrMPs. The technique has the potential to become an important tool for diagnosis and prognosis of breast and other cancers.
Download the full text: PDF | HTML
