OBOC Small-Molecule Combinatorial
Library Encoded by Halogenated
Mass-Tags
Sung Hee Hwang,Alan Lehman,Xin Cong,Marilyn M. Olmstead,#
Kit S. Lam,Carlito B. Lebrilla, andMark J. Kurth*
Department of Chemistry, University of California-Davis, One Shields Avenue,
Davis, California 95616-5295, and Division of Hematology and Oncology,
Department of Internal Medicine, University of California-Davis Cancer Center,
4501 X Street, Sacramento, California 95817
mjkurth@ucdavis.edu
Received August 11, 2004
Abstract:
A bromine-/chlorine-containing mass-tag encoding strategy for a small-molecule OBOC combinatorial library is reported. The resulting MALDI
FTMS isotope pattern of each tag clearly defines the component building blocks of each "hit" bead in an 1890-member demonstration library
screened on-bead for binding against streptavidin via both enzyme-linked colorimetric and Quantum Dot/COPAS assays.
