Response to “Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Based Proteomics Method”
- Brahim Achour*Brahim Achour*E-mail: [email protected]Centre for Applied Pharmacokinetic Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, U.K.More by Brahim Achour
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- Amin Rostami-HodjeganAmin Rostami-HodjeganCentre for Applied Pharmacokinetic Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, U.K.Simcyp Ltd. (a Certara company), 1 Concourse Way, Sheffield S1 2BJ, U.K.More by Amin Rostami-Hodjegan
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- Jill Barber*Jill Barber*E-mail: [email protected]Centre for Applied Pharmacokinetic Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, U.K.More by Jill Barber
Abstract
A recent publication in this journal reported the application of a targeted proteomic strategy using a quantitative concatemer (QconCAT) standard to the assessment of allele-specific expression of UGT2B15 claiming this methodology to be a “novel” approach (J. Proteome Res.2018, 17 (10), 3606–3612, DOI: 10.1021/acs.jproteome.8b00620). While the application is not common, the method was previously described and reported by our group in relation to the quantification of CYP2B6 alleles (J. Proteome Res.2013, 12 (12), 5934–5942, DOI: 10.1021/pr400279u) to assess the expression of a prevalent polymorphism in a Caucasian population.
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