A Chemiluminescence Reaction between Hydrogen Peroxide and Acetonitrile and Its Applications

Jianzhong Lu, Choiwan Lau, Mikio Morizono, Kazuko Ohta, and Masaaki Kai*
School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan
Anal. Chem., 2001, 73 (24), pp 5979–5983
DOI: 10.1021/ac010688d
Publication Date (Web): November 9, 2001
Copyright © 2001 American Chemical Society

Abstract

Here we report a new chemiluminescence reaction between basic aqueous H2O2 and acetonitrile. Its ultraweak chemiluminescence could be greatly enhanced by luminol, isoluminol-labeled streptavidin, and an Edman-type fluorescent reagent. Light emission was intense and long-lived, and this facilitated the initiation of the reaction and the measurement of the light emission. The present results permit us to propose a series of convenient, highly sensitive, and enzyme-free techniques for the detection and quantification of luminol, related conjugates, acetonitrile, and amino acids. Overall, this new chemiluminescence reaction will be quite promising for numerous applications in immunoassay, DNA hybridization, environmental monitoring. and postcolumn chromatographic detection.

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History

  • Published In Issue December 15, 2001
  • Received for review June 19, 2001. Accepted September 23, 2001.

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