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Article

Collection of Trace Amounts of DNA/mRNA Molecules Using Genomagnetic Nanocapturers

Xiaojun Zhao, Rovelyn Tapec-Dytioco, Kemin Wang,^† and Weihong Tan*

Center for Research at the Bio/nano Interface, Department of Chemistry and the McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200

Anal. Chem., 2003, 75 (14), pp 3476–3483

DOI: 10.1021/ac034330o

Publication Date (Web): June 3, 2003

Abstract

The collection and then the separation of rare DNA/mRNA targets with single-base mismatches in a complex matrix is critically important in human disease diagnostics, gene expression studies, and gene profiling. The major result of this work is the development and application of a novel genomagnetic nanocapturer (GMNC) for the collection, separation, and detection of trace amounts of DNA/RNA molecules with one single-base difference. The GMNC is constructed by bioconjugating molecular beacon DNA probes onto magnetic nanoparticle surfaces. We have successfully applied the GMNC in artificial buffer solution samples and in cancer cell samples, both containing different proteins and random DNA sequences. Our method has three distinctly useful features: highly efficient collection of trace amount of DNA/mRNA samples down to femtomolar (10^-¹⁵ M) concentrations; excellent ability to differentiate single-base-mismatched DNA/mRNA samples by combining the exceptional specificity of molecular beacons and the separation power of magnetic nanoparticles; and real-time monitoring and confirmation of the collected gene products. The newly developed genomagnetic nanocapturers will be highly useful for the collection of trace amounts of DNA/mRNA targets in a variety of sample sources in forensic, medical, and biotechnological fields.

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