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Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Section:Abstract
Mass-selected peptide ions produced by electrospray ionization were deposited onto fluorinated self-assembled monolayer surfaces (FSAM) surfaces by soft landing using a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) specially designed for studying interactions of large ions with surfaces. Analysis of the modified surface was performed in situ by combining 2-keV Cs+ secondary ion mass spectrometry with FT-ICR detection of the sputtered ions (FT-ICR-SIMS). Regardless of the initial charge state of the precursor ion, the SIMS mass spectra included singly protonated peptide ion, peptide fragment ions, and peaks characteristic of the surface in all cases. In some experiments, multiply protonated peptide ions and [M + Au]+ ions were also observed upon SIMS analysis of modified surfaces. For comparison with the in situ analysis of the modified surfaces, ex situ analysis of some of the modified surfaces was performed by 25-keV Ga+ time-of-flight−secondary ion mass spectrometry (TOF-SIMS). The ex situ analysis demonstrated that a significant number of soft-landed peptide ions remain charged on the surface even when exposed to air for several hours after deposition. Charge retention of soft-landed ions dramatically increases the ion yields obtained during SIMS analysis and enables very sensitive detection of deposited material at less than 1% of monolayer coverage. Accumulation of charged species on the surface undergoes saturation due to coulomb repulsion between charges at close to 30% coverage. We estimated that close to 1 ng of peptide could be deposited on the spot area of 4 mm2 of the FSAM surface without reaching saturation.
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This article has been cited by 10 ACS Journal articles (5 most recent appear below).
Charge Retention by Gold Clusters on Surfaces Prepared Using Soft Landing of Mass Selected Ions
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Grant E. Johnson, Thomas Priest, and Julia LaskinACS Nano2012 6 (1), 573-582Monodisperse gold clusters have been prepared on surfaces in different charge states through soft landing of mass-selected ions. Ligand-stabilized gold clusters were prepared in methanol solution by reduction of chloro(triphenylphosphine)gold(I) with ...
In Situ Reactivity and TOF-SIMS Analysis of Surfaces Prepared by Soft and Reactive Landing of Mass-Selected Ions
Grant E. Johnson, Michael Lysonski and Julia LaskinAnalytical Chemistry2010 82 (13), 5718-5727In Situ Reactivity and TOF-SIMS Analysis of Surfaces Prepared by Soft and Reactive Landing of Mass-Selected Ions
Grant E. Johnson, Michael Lysonski and Julia LaskinAnalytical Chemistry2010 82 (13), 5718-5727An instrument has been designed and constructed that enables in situ reactivity and time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis of surfaces prepared or modified through soft and reactive landing of mass-selected polyatomic cations ...
Soft-Landing of CoIII(salen)+ and MnIII(salen)+ on Self-Assembled Monolayer Surfaces
Julia Laskin, Peng Wang and Omar HadjarThe Journal of Physical Chemistry C2010 114 (12), 5305-5311Soft-Landing of CoIII(salen)+ and MnIII(salen)+ on Self-Assembled Monolayer Surfaces
Julia Laskin, Peng Wang and Omar HadjarThe Journal of Physical Chemistry C2010 114 (12), 5305-5311Soft-landing of mass-selected CoIII(salen)+ and MnIII(salen)+ complexes [salen = N,N′-ethylenebis(salicylideneaminato)] was performed using self-assembled monolayer surfaces of alkanethiol (HSAM) and fluorinated alkanethiol (FSAM) on gold as targets. ...
In situ Studies of Soft- and Reactive Landing of Mass-Selected Ions Using Infrared Reflection Absorption Spectroscopy
Qichi Hu, Peng Wang, Paul L. Gassman and Julia LaskinAnalytical Chemistry2009 81 (17), 7302-7308In situ Studies of Soft- and Reactive Landing of Mass-Selected Ions Using Infrared Reflection Absorption Spectroscopy
Qichi Hu, Peng Wang, Paul L. Gassman and Julia LaskinAnalytical Chemistry2009 81 (17), 7302-7308Grazing incidence infrared reflection absorption spectroscopy (IRRAS) for in situ and in real time characterization of substrates modified by soft and reactive landing (SL and RL) of complex ions was implemented on a mass-selected ion deposition ...
Ion Soft Landing Using a Rectilinear Ion Trap Mass Spectrometer
Wen-Ping Peng, Michael P. Goodwin, Zongxiu Nie, Michael Volný, Zheng Ouyang and R. Graham CooksAnalytical Chemistry2008 80 (17), 6640-6649Ion Soft Landing Using a Rectilinear Ion Trap Mass Spectrometer
Wen-Ping Peng, Michael P. Goodwin, Zongxiu Nie, Michael Volný, Zheng Ouyang and R. Graham CooksAnalytical Chemistry2008 80 (17), 6640-6649A new ion soft landing instrument has been built for the controlled deposition of mass selected polyatomic ions. The instrument has been operated with an electrospray ionization source; its major components are an electrodynamic ion funnel to reduce ion ...
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History
- Published In Issue June 01, 2005
- Received for review December 17, 2004. Accepted April 7, 2005.
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