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Preparative Linear Ion Trap Mass Spectrometer for Separation and Collection of Purified Proteins and Peptides in Arrays Using Ion Soft Landing
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Section:Abstract
A preparative mass spectrometer for microarray fabrication is reported. The instrument includes an atmospheric pressure ionization source, a linear ion trap mass analyzer, an ion collection surface positioning system, and a surface loading chamber with independent vacuum pumping. It was designed for the production of protein arrays using the ion soft-landing technique to collect ions on a surface after separation by mass/charge ratio. Small microarrays have been prepared by isolating and soft landing individual protein or peptide ions after electrospray ionization of mixtures. The composition and purity of the separated materials has been confirmed using independent external mass spectrometric analysis of rinse solutions of the collected spots, either by the new method of electrosonic spray ionization MS or by nanospray ionization MS. The ability to retain bioactivity in the mass-selected and collected biomolecules has been demonstrated in particular cases. The reported instrument has also been characterized as an analytical mass spectrometer.
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This article has been cited by 15 ACS Journal articles (5 most recent appear below).
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In Situ Reactivity and TOF-SIMS Analysis of Surfaces Prepared by Soft and Reactive Landing of Mass-Selected Ions
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Grant E. Johnson, Michael Lysonski and Julia LaskinAnalytical Chemistry2010 82 (13), 5718-5727An instrument has been designed and constructed that enables in situ reactivity and time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis of surfaces prepared or modified through soft and reactive landing of mass-selected polyatomic cations ...
Soft-Landing of CoIII(salen)+ and MnIII(salen)+ on Self-Assembled Monolayer Surfaces
Julia Laskin, Peng Wang and Omar HadjarThe Journal of Physical Chemistry C2010 114 (12), 5305-5311Soft-Landing of CoIII(salen)+ and MnIII(salen)+ on Self-Assembled Monolayer Surfaces
Julia Laskin, Peng Wang and Omar HadjarThe Journal of Physical Chemistry C2010 114 (12), 5305-5311Soft-landing of mass-selected CoIII(salen)+ and MnIII(salen)+ complexes [salen = N,N′-ethylenebis(salicylideneaminato)] was performed using self-assembled monolayer surfaces of alkanethiol (HSAM) and fluorinated alkanethiol (FSAM) on gold as targets. ...
Mass Selection of Ions from Beams Using Waveform Isolation in Radiofrequency Quadrupoles
Qingyu Song, Scott A. Smith, Liang Gao, Wei Xu, Michael Volný, Zheng Ouyang and R. Graham CooksAnalytical Chemistry2009 81 (5), 1833-1840Mass Selection of Ions from Beams Using Waveform Isolation in Radiofrequency Quadrupoles
Qingyu Song, Scott A. Smith, Liang Gao, Wei Xu, Michael Volný, Zheng Ouyang and R. Graham CooksAnalytical Chemistry2009 81 (5), 1833-1840A waveform isolation method is described for the mass-selective transmission of ions through quadrupole mass filters, and it is implemented on a new tandem mass analyzer instrument. The method features the application of broad-band waveforms comprising ...
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History
- Published In Issue November 01, 2004
- Received for review July 12, 2004. Accepted August 16, 2004.
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