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Article

Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition

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Dominik Schwudke,^† Jeffrey Oegema,^‡ Lyle Burton,^§ Eugeni Entchev,^† J. Thomas Hannich,^† Christer S. Ejsing,^† Teymuras Kurzchalia,^† and Andrej Shevchenko*^†

MPI of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany, Scionics Computer Innovation GmbH, Tatzberg 47-51, 01307 Dresden, Germany, and MDS Sciex, Concord, 71 Four Valley Drive, L4K 4V8 Concord, Canada

Anal. Chem., 2006, 78 (2), pp 585–595

DOI: 10.1021/ac051605m

Publication Date (Web): December 7, 2005

†

MPI of Molecular Cell Biology and Genetics.

‡

Scionics Computer Innovation GmbH.

MDS Sciex.

Corresponding author: (e-mail) shevchenko@mpi-cbg.de.

Abstract

Data-dependent acquisition of MS/MS spectra from lipid precursors enables to emulate the simultaneous acquisition of an unlimited number of precursor and neutral loss scans in a single analysis. This approach takes full advantage of rich fragment patterns in tandem mass spectra of lipids and enables their profiling by complex (Boolean) scans, in which masses of several fragment ions are considered within a single logical framework. No separation of lipids is required, and the accuracy of identification and quantification is not compromised, compared to conventional precursor and neutral loss scanning.

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History

Published In Issue January 15, 2006
Received for review September 8, 2005. Accepted November 1, 2005.

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Article

Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition

Abstract

Tools

SciFinder Links

History

Recommend & Share

Related Content

Automated Identification and Quantification of Glycerophospholipid Molecular Species by Multiple Precursor Ion Scanning

Top-Down Lipidomic Screens by Multivariate Analysis of High-Resolution Survey Mass Spectra

Quantitative Profiling of Phospholipids by Multiple Precursor Ion Scanning on a Hybrid Quadrupole Time-of-Flight Mass Spectrometer

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