Same-Single-Cell Analysis for the Study of Drug Efflux Modulation of Multidrug Resistant Cells Using a Microfluidic Chip

XiuJun Li†, Victor Ling‡ and Paul C. H. Li*†
Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada, and BC Cancer Research Center, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada
Anal. Chem., 2008, 80 (11), pp 4095–4102
DOI: 10.1021/ac800231k
Publication Date (Web): April 30, 2008
Copyright © 2008 American Chemical Society
†

Simon Fraser University.

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‡

BC Cancer Research Center.

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* Corresponding author. E-mail: paulli@sfu.ca. Phone: 778-782-5956 . Fax: 778-782-3765.

Abstract

Since multidrug resistance (MDR) is a major cause of failure in cancer chemotherapy, we report a microfluidic approach combined with the same-single-cell analysis to investigate the modulation of MDR, manifested as the inhibition of drug efflux. A microfluidic chip that was capable of selecting and retaining a single multidrug-resistant cancer cell was used to investigate drug efflux inhibition in leukemia cell lines. Three advantages of the microfluidic-based same-single-cell analysis (dubbed as SASCA) method have been revealed. First, it readily detects the modulation of drug efflux of anticancer compounds (e.g., daunorubicin) by MDR modulators (e.g., verapamil) among cellular variations. Second, SASCA is able to compare the different cellular abilities in response to drug efflux modulation based on the drug transport kinetics of single cells. Third, SASCA requires only a small number of cells, which may be beneficial for investigating drug resistance in minor cell subpopulations (e.g., cancer “stem” cells).

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History

  • Published In Issue June 01, 2008
  • Article ASAPApril 30, 2008
  • Received: February 01, 2008
    Accepted: March 21, 2008

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