Prev. Article Next Article Table of Contents

Article

Quantification in Comprehensive Two-Dimensional Liquid Chromatography

Luigi Mondello*^†^‡, Miguel Herrero^†, Tiina Kumm^†, Paola Dugo^§, Hernan Cortes and Giovanni Dugo^†

Dipartimento Farmaco-chimico, Facolt

di Farmacia, Universit

di Messina, Viale Annunziata, 98168, Messina, Italy, Campus-Biomedico, Via E. Longoni 47, 00155, Rome, Italy, Dipartimento di Scienze degli Alimenti e dell’Ambiente, Facolt

di Scienze, Universit

di Messina, Salita Sperone 31, 98166, Messina, Italy, and The Dow Chemical Company, Analytical Sciences, 1897 Building, Midland, Michigan, 48667

Anal. Chem., 2008, 80 (14), pp 5418–5424

DOI: 10.1021/ac800484y

Publication Date (Web): June 3, 2008

* Corresponding author. Phone: +39 090 6766536. Fax: +39 090 6766532. E-mail: lmondello@unime.it.

†

Dipartimento Farmaco-chimico, Universit di Messina.

‡

Campus-Biomedico.

Dipartimento di Scienze degli Alimenti e dell’Ambiente, Universit di Messina.

The Dow Chemical Company.

Abstract

Although the use of comprehensive two-dimensional liquid chromatography (LC×LC) as a powerful separation technique is continuously increasing, its employment in quantification experiments is rather limited. The present research is focused on the quantification of a series of standards, as well as real-world sample compounds, by using dedicated laboratory-constructed LC×LC software, developed through a novel approach. Moreover, the difficulties encountered during software operation, in various elution conditions, are described and discussed. The results attained were compared with those observed in conventional LC, and no statistically significant differences were observed in the determination of aurapten in grapefruit oil. However, a loss in sensitivity was observed when using LC×LC (limit of detection = 0.10 ppm) compared to conventional LC (limit of detection = 0.05 ppm) as a consequence of the sample dilution in comprehensive two-dimensional liquid chromatography.

View: Full Text HTML | Hi-Res PDF | PDF w/ Links