Self-Assembly of Quantum Dots and Carbon Nanotubes for Ultrasensitive DNA and Antigen Detection

Daxiang Cui*†‡, Bifeng Pan†, Hong Zhang‡, Feng Gao†, Rina Wu§, Jingping Wang‡, Rong He† and Toru Asahi‡
Department of Bio-nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, 800Dongchuan Road, Shanghai 200240,P.R. China, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Wasedatsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan, Department of Dermatology of First Affiliated Hospital of Inner Mongolia Medical College, Huhehaote, 010050, P. R. China
Anal. Chem., 2008, 80 (21), pp 7996–8001
DOI: 10.1021/ac800992m
Publication Date (Web): September 25, 2008
Copyright © 2008 American Chemical Society
* Corresponding author. E-mail: dxcui@sjtu.edu.cn.
,
†

Shanghai JiaoTong University.

,
‡

Waseda University.

,
§

First Affiliated Hospital of Inner Mongolia Medical College.

Abstract

A highly selective, ultrasensitive, fluorescence detection method for DNA and antigen based on self-assembly of multiwalled carbon nanotubes (CNTs) and CdSe quantum dots (QDs) via oligonucleotide hybridization is reported. Mercaptoalkyloligonucleotide molecules bind to the quantum dots, while amineoalkyloligonucleotides bind to CNTs with −COCl surface groups. QDs and CNTs further assemble into nanohybrids through DNA hybridization in the presence of target complementary oligonucleotides. The method is achieved with good repeatability with the detection limit of 0.2 pM DNA molecules and 0.01 nM antigen molecules. This novel detection system can also be used for multicomponent detection and antigen−antibody immunoreaction. The novel system has great potential in applications such as ultrasensitive pathogen DNA or antigen or antibody detection, molecular imaging, and photoelectrical biosensors.

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History

  • Published In Issue November 01, 2008
  • Article ASAPSeptember 25, 2008
  • Received: May 14, 2008
    Accepted: August 22, 2008

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