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Digitized Molecular Diagnostics: Reading Disk-Based Bioassays with Standard Computer Drives

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Yunchao Li, Lily M. L. Ou and Hua-Zhong Yu*

Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada

Anal. Chem., 2008, 80 (21), pp 8216–8223

DOI: 10.1021/ac8012434

Publication Date (Web): September 26, 2008

* To whom correspondence should be addressed. E-mail: hogan_yu@sfu.ca. Fax: (778) 782-3765.

Section:

Biochemical Methods

Abstract

We report herein a digital signal readout protocol for screening disk-based bioassays with standard optical drives of ordinary desktop/notebook computers. Three different types of biochemical recognition reactions (biotin−streptavidin binding, DNA hybridization, and protein−protein interaction) were performed directly on a compact disk in a line array format with the help of microfluidic channel plates. Being well-correlated with the optical darkness of the binding sites (after signal enhancement by gold nanoparticle-promoted autometallography), the reading error levels of prerecorded audio files can serve as a quantitative measure of biochemical interaction. This novel readout protocol is about 1 order of magnitude more sensitive than fluorescence labeling/scanning and has the capability of examining multiplex microassays on the same disk. Because no modification to either hardware or software is needed, it promises a platform technology for rapid, low-cost, and high-throughput point-of-care biomedical diagnostics.

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Citing Articles

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This article has been cited by 8 ACS Journal articles (5 most recent appear below).

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