Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects

Paul C. H. Li and D. Jed Harrison*
Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2
Anal. Chem., 1997, 69 (8), pp 1564–1568
DOI: 10.1021/ac9606564
Publication Date (Web): April 15, 1997
Copyright © 1997 American Chemical Society
*

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

Abstract

A microfluidic system was fabricated on a glass chip to study mobilization of biological cells on-chip. Electroosmotic and/or electrophoretic pumping were used to drive the cell transport within a network of capillary channels. Whole cells such as Saccharomyces cerevisiae, canine erythrocyte, and Escherichia coli were employed in this work. Photographs are presented to illustrate how cells are selected and transported from one location to another within the capillary network, with velocities up to about 0.5 mm/s in capillaries with a 15- × 55-μm cross section. The mixing of canine erythrocytes with the lysing agent sodium dodecyl sulfate, at an intersection within the chip, was performed to demonstrate that cell selection and subsequent reaction can be accomplished within the microchip.

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History

  • Published In Issue April 15, 1997
  • Received for review July 3, 1996. Accepted January 21, 1997.

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