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Article

Localized Avidin/Biotin Derivatization of Glassy Carbon Electrodes Using SECM

Wilbur B. Nowall,^‡ David O. Wipf,^† and Werner G. Kuhr*^‡

Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, and Department of Chemistry, University of California, Riverside, California 92521

Anal. Chem., 1998, 70 (13), pp 2601–2606

DOI: 10.1021/ac971310b

Publication Date (Web): May 9, 1998

‡

University of California.

†

Mississippi State University.

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

Abstract

Different forms of the microreagent mode of SECM were used to attach biotin or make “clean” spots on micron-sized regions on the surface of a carbon electrode. In the direct-write mode, the SECM probe tip is used as an electrochemical “pen” depositing biotin in micron-sized lines on the carbon substrate as it is scanned across its surface. In the negative microreagent mode, the SECM probe tip is used as an electrochemical “eraser” cleaning off the surface attached molecules and leaving clean spots on the surface of a globally derivatized carbon surface. This type of simple micromodification of the surface of a carbon electrode will allow the fabrication of biosensors that can potentially be tailor-made for a variety of applications.

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