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Nanobowl-Supported Liposomes Improve Drug Loading and Delivery

  • Zhong-Jian Chen
    Zhong-Jian Chen
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Si-Cong Yang
    Si-Cong Yang
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Si-Cong Yang
  • Xue-Liang Liu
    Xue-Liang Liu
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Yuhao Gao
    Yuhao Gao
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Yuhao Gao
  • Xiao Dong
    Xiao Dong
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Xiao Dong
  • Xing Lai
    Xing Lai
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Xing Lai
  • Mao-Hua Zhu
    Mao-Hua Zhu
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Mao-Hua Zhu
  • Hai-Yi Feng
    Hai-Yi Feng
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Hai-Yi Feng
  • Xin-Di Zhu
    Xin-Di Zhu
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Xin-Di Zhu
  • Qin Lu
    Qin Lu
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    More by Qin Lu
  • Mei Zhao
    Mei Zhao
    Department of Pharmacy, Shanghai University of Medicine and Health Sciences, 279 Zhouzhu Road, Shanghai 201318, China
    More by Mei Zhao
  • Hong-Zhuan Chen
    Hong-Zhuan Chen
    Institute of Interdisciplinary Integrative Biomedical Research, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Jonathan F. Lovell*
    Jonathan F. Lovell
    Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, United States
    *Email: [email protected] (J.F.L.).
  • , and 
  • Chao Fang*
    Chao Fang
    Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    *Email: [email protected] (C.F.).
    More by Chao Fang
Cite this: Nano Lett. 2020, 20, 6, 4177–4187
Publication Date (Web):May 20, 2020
https://doi.org/10.1021/acs.nanolett.0c00495

Copyright © 2020 American Chemical Society. This publication is licensed under these Terms of Use.

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Abstract

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Liposomal drug delivery for cancer therapy can be limited due to drug leakage in circulation. Here, we develop a new method to enhance the stability of actively loaded liposomal doxorubicin (DOX) through embedding a stiff nanobowl in the liposomal water cavity. Nanobowl-supported liposomal DOX (DOX@NbLipo) resists the influence of plasma protein and blood flow shear force to prevent drug leakage. This approach yields improved drug delivery to tumor sites and enhanced antitumor efficacy. Compared to alternative methods of modifying liposome surface and composition for stability, this approach designs a physical support for an all-aqueous nanoliposomal cavity. Nanobowl stabilization of liposomes is a simple and effective method to improve carrier stability and drug delivery.

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The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00495.

  • Details of experimental materials and methods; characterization of the nanoparticles; cell uptake and cytotoxicity; in vivo biodistribution and pharmacokinetics; histopathological examination; serum biochemistry, complete blood panel and hemolysis analysis; increase in life span (ILS); Figures S1–S24 and Tables S1–S2 (PDF)

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