Article
New Triphosphate Conjugates Bearing Reporter Groups: Labeling of DNA Fragments for Microarray Analysis
Corresponding author. Tel: 7-495 135-2255; fax: 7-495-135-1405; e-mail: kukhan86@hotmail.com.
Abstract
A simple and convenient method for incorporation of fluorescent or ligand groups into 3‘-termini of DNA fragments is proposed. A set of triphosphoric acid monoesters bearing fluorescent groups or biotin attached to the triphosphate fragment through linkers of different lengths and structures was synthesized. All the compounds were substrates for calf thymus terminal deoxynucleotidyltransferase and were used for incorporation of marker groups into 3‘-termini of DNA fragments. The compounds were successfully applied for DNA labeling during post-PCR target preparation for microarray analysis.
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History
- Published In Issue May 16, 2007
- Received July 14, 2006
Revised Manuscript Received January 17, 2007
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