New Triphosphate Conjugates Bearing Reporter Groups:  Labeling of DNA Fragments for Microarray Analysis

Ludmila A. Alexandrova, Maxim V. Jasko, Elena E. Belobritskaya, Alexander V. Chudinov, Olga N. Mityaeva, Tatyana V. Nasedkina, Alexander S. Zasedatelev, and Marina K. Kukhanova*
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, Moscow 119991, Russian Federation
Bioconjugate Chem., 2007, 18 (3), pp 886–893
DOI: 10.1021/bc060213z
Publication Date (Web): March 13, 2007
Copyright © 2007 American Chemical Society
*

 Corresponding author. Tel:  7-495 135-2255; fax:  7-495-135-1405; e-mail:  kukhan86@hotmail.com.

Abstract

A simple and convenient method for incorporation of fluorescent or ligand groups into 3‘-termini of DNA fragments is proposed. A set of triphosphoric acid monoesters bearing fluorescent groups or biotin attached to the triphosphate fragment through linkers of different lengths and structures was synthesized. All the compounds were substrates for calf thymus terminal deoxynucleotidyltransferase and were used for incorporation of marker groups into 3‘-termini of DNA fragments. The compounds were successfully applied for DNA labeling during post-PCR target preparation for microarray analysis.

Tools

History

  • Published In Issue May 16, 2007
  • Received July 14, 2006
    Revised Manuscript Received January 17, 2007

Recommend & Share

Related Content

Other ACS content by these authors: