Cart
Article
The Missing Link in the Fungal l-Arabinose Catabolic Pathway, Identification of the l-Xylulose Reductase Gene†,‡
Full Text HTML
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Section:Abstract
The fungal l-arabinose pathway consists of five enzymes, aldose reductase, l-arabinitol 4-dehydrogenase, l-xylulose reductase, xylitol dehydrogenase, and xylulokinase. All the genes encoding the enzymes of this pathway are known except for that of l-xylulose reductase (EC 1.1.1.10). We identified a gene encoding this enzyme from the filamentous fungus Trichoderma reesei (Hypocrea jecorina). The gene was named lxr1. It was overexpressed in the yeast Saccharomyces cerevisiae, and the enzyme activity was confirmed in a yeast cell extract. Overexpression of all enzymes of the l-arabinose pathway in S. cerevisiae led to growth of S. cerevisiae on l-arabinose; i.e., we could show that the pathway is active in a heterologous host. The lxr1 gene encoded a protein with 266 amino acids and a calculated molecular mass of 28 428 Da. The LXRI protein is an NADPH-specific reductase. It has activity with l-xylulose, d-xylulose, d-fructose, and l-sorbose. The highest affinity is toward l-xylulose (Km = 16 mM). In the reverse direction, we found activity with xylitol, d-arabinitol, d-mannitol, and d-sorbitol. It requires a bivalent cation for activity. It belongs to the protein family of short chain dehydrogenases. The enzyme is catalytically similar and homologous in sequence to a d-mannitol:NADP 2-dehydrogenase (EC 1.1.1.138).
Citing Articles
Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.
This article has been cited by 5 ACS Journal articles (5 most recent appear below).
Solution Structure and Functional Characterization of Jingzhaotoxin-XI: A Novel Gating Modifier of both Potassium and Sodium Channels,
Zhi Liao, Chunhua Yuan, Meichun Deng, Jiang Li, Jinjun Chen, Yuejun Yang, Weijun Hu, and Songping LiangBiochemistry2006 45 (51), 15591-15600Solution Structure and Functional Characterization of Jingzhaotoxin-XI: A Novel Gating Modifier of both Potassium and Sodium Channels,
Zhi Liao, Chunhua Yuan, Meichun Deng, Jiang Li, Jinjun Chen, Yuejun Yang, Weijun Hu, and Songping LiangBiochemistry2006 45 (51), 15591-15600JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I−IV, II−V, and III−VI. Using 3‘- and 5‘-RACE methods, ...
Genetic Engineering of S. cerevisiae for Pentose Utilization
Peter Richard, Ritva Verho, John Londesborough and Merja Penttilä2006 921 (), 184-192Genetic Engineering of S. cerevisiae for Pentose Utilization
Peter Richard, Ritva Verho, John Londesborough and Merja Penttilä2006 921 (), 184-192Recombinant Saccharomyces cerevisiae, able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields. Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ...
Identification in the Mold Hypocrea jecorina of the First Fungal d-Galacturonic Acid Reductase
Satu Kuorelahti, Nisse Kalkkinen, Merja Penttilä, John Londesborough, and Peter RichardBiochemistry2005 44 (33), 11234-11240Identification in the Mold Hypocrea jecorina of the First Fungal d-Galacturonic Acid Reductase
Satu Kuorelahti, Nisse Kalkkinen, Merja Penttilä, John Londesborough, and Peter RichardBiochemistry2005 44 (33), 11234-11240A d-galacturonic acid reductase and the corresponding gene were identified from the mold Hypocrea jecorina (Trichoderma reesei). We hypothesize that the enzyme is part of a fungal d-galacturonic acid catabolic pathway which has not been described ...
Commodity Chemicals Production by Fermentation: An Overview
Badal C. Saha2003 862 (), 3-17Commodity Chemicals Production by Fermentation: An Overview
Badal C. Saha2003 862 (), 3-17Various commodity chemicals such as alcohols, polyols, organic acids, amino acids, polysaccharides, biodegradable plastic components, and industrial enzymes can be produced by fermentation. This overview focuses on recent research progress in the ...
Identification of the First Fungal NADP-GAPDH from Kluyveromyces lactis
Ritva Verho, Peter Richard, Per Harald Jonson, Lena Sundqvist, John Londesborough, and Merja PenttiläBiochemistry2002 41 (46), 13833-13838Identification of the First Fungal NADP-GAPDH from Kluyveromyces lactis
Ritva Verho, Peter Richard, Per Harald Jonson, Lena Sundqvist, John Londesborough, and Merja PenttiläBiochemistry2002 41 (46), 13833-13838Deletion of the phosphoglucose isomerase gene, PGI1, in Saccharomyces cerevisiae leads to a phenotype for which glucose is toxic. This is related to overproduction of NADPH through the oxidative part of the pentose phosphate pathway and the incompetence ...
Tools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
Accession Codes
History
- Published In Issue May 21, 2002
- Received January 9, 2002
Revised Manuscript Received February 28, 2002
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
