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The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif^†

Ricardo Medina, Timothy Buck, Sayyed K. Zaidi, Angela Miele-Chamberland, Jane B. Lian, Janet L. Stein, Andre J. van Wijnen and Gary S. Stein*

Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Biochemistry, 2008, 47 (44), pp 11415–11423

DOI: 10.1021/bi800961d

Publication Date (Web): October 14, 2008

†

These studies were supported by NIH Grant GM032010.

* To whom correspondence should be addressed: Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Ave. N., Worcester, MA 01655. Telephone: (508) 856-5625. Fax: (508) 856-6800. E-mail: Gary.Stein@umassmed.edu.

Abstract

Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of human histone gene expression at the G1−S phase transition plays a critical role. The zinc finger HiNF-P and coactivator p220^NPAT proteins are key regulators of histone gene expression. Here, we describe a novel HiNF-P-specific conserved region (PSCR) located within the C-terminus that is present in HiNF-P homologues of all metazoan species that have been examined. The PSCR motif is required for activation of histone H4 gene transcription and contributes to DNA binding of HiNF-P. Thus, the PSCR module represents an auxiliary DNA-binding determinant that plays a critical role in mediating histone gene expression during the cell cycle and defines HiNF-P as a unique cell cycle regulatory member of the zinc finger transcription factor family.

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History

Published In Issue November 04, 2008
Article ASAPOctober 14, 2008
Received: May 21, 2008
Revised: August 15, 2008

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Article

The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif^†