Importance of the Conserved Lysine 83 Residue of Zea mays Cytochrome b561 for Ascorbate-Specific Transmembrane Electron Transfer As Revealed by Site-Directed Mutagenesis Studies

Nobuyuki Nakanishi, Motiur Md. Rahman, Yoichi Sakamoto, Tadakazu Takigami, Kazuo Kobayashi, Hiroshi Hori#, Toshiharu Hase, Sam-Yong Park◊ and Motonari Tsubaki*
‡ Department of Molecular Science and Material Engineering, Graduate School of Science and Technology
§ Department of Chemistry, Graduate School of Science
Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan
Department of Life Science, Graduate School of Science, Himeji Institute of Technology, Kamigoori-cho, Akou-gun, Hyogo 678-1297, Japan
Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan
# Division of Bioengineering, Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan
◊ Protein Design Laboratory, Division of Science of Biological Supramolecular Systems, Graduate School of Integrated Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan
Biochemistry, 2009, 48 (44), pp 10665–10678
DOI: 10.1021/bi9010682
Publication Date (Web): October 5, 2009
Copyright © 2009 American Chemical Society
*Corresponding author. Tel: +81-78-803-6582. Fax: +81-78-803-6582. E-mail: mtsubaki@kobe-u.ac.jp.

Abstract

Abstract Image

Cytochromes b561, a novel class of transmembrane electron transport proteins residing in a large variety of eukaryotic cells, have a number of common structural features including six hydrophobic transmembrane α-helices and two heme ligation sites. We found that recombinant Zea mays cytochrome b561 obtained by a heterologous expression system using yeast Pichia pastoris cells could utilize the ascorbate/mondehydroascorbate radical as a physiological electron donor/acceptor. We found further that a concerted proton/electron transfer mechanism might be operative in Z. mays cytochrome b561 as well upon the electron acceptance from ascorbate to the cytosolic heme center. The well-conserved Lys83 residue in a cytosolic loop was found to have a very important role(s) for the binding of ascorbate and the succeeding electron transfer via electrostatic interactions based on the analyses of three site-specific mutants, K83A, K83E, and K83D. Further, unusual behavior of the K83A mutant in pulse radiolysis experiments indicated that Lys83 might also be responsible for the intramolecular electron transfer to the intravesicular heme. On the other hand, pulse radiolysis experiments on two site-specific mutants, S118A and W122A, for the well-conserved residues in the putative monodehydroascorbate radical binding site showed that their electron transfer activities to the monodehydroascorbate radical were very similar to those of the wild-type protein, indicating that Ser118 and Trp122 do not have major roles for the redox events on the intravesicular side.

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History

  • Published In Issue November 10, 2009
  • Article ASAPOctober 16, 2009
  • Just Accepted ManuscriptOctober 05, 2009
  • Received: June 26, 2009
    Revised: October 04, 2009

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