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Multivalent Display of Proteins on Viral Nanoparticles Using Molecular Recognition and Chemical Ligation Strategies

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P. Arno Venter†, Anouk Dirksen‡, Diane Thomas§, Marianne Manchester§, Philip E. Dawson‡, and Anette Schneemann*†

^†Department of Molecular Biology and ^‡Departments of Cell Biology and Chemistry, The Scripps Research Institute, La Jolla, California 92037, United States

Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, California 92093, United States

Biomacromolecules, 2011, 12 (6), pp 2293–2301

DOI: 10.1021/bm200369e

Publication Date (Web): May 6, 2011

Phone: (858) 784-8643. Fax: (858) 784-7979. E-mail: aschneem@scripps.edu.

Section:

Biochemical Methods

Abstract

Multivalent display of heterologous proteins on viral nanoparticles forms a basis for numerous applications in nanotechnology, including vaccine development, targeted therapeutic delivery, and tissue-specific bioimaging. In many instances, precise placement of proteins is required for optimal functioning of the supramolecular assemblies, but orientation- and site-specific coupling of proteins to viral scaffolds remains a significant technical challenge. We have developed two strategies that allow for controlled attachment of a variety of proteins on viral particles using covalent and noncovalent principles. In one strategy, an interaction between domain 4 of anthrax protective antigen and its receptor was used to display multiple copies of a target protein on virus-like particles. In the other, expressed protein ligation and aniline-catalyzed oximation was used to display covalently a model protein. The latter strategy, in particular, yielded nanoparticles that induced potent immune responses to the coupled protein, suggesting potential applications in vaccine development.

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