Electrospinning of Bovine Serum Albumin. Optimization and the Use for Production of Biosensors.

Tomasz Kowalczyk*†, Aleksandra Nowicka‡, Danek Elbaum‡ and Tomasz A. Kowalewski†
Institute of Fundamental Technological Research, Polish Academy of Sciences (IPPT PAN), Swietokrzyska 21, Warszawa, 00-049, Poland, and Institute of Physics, Polish Academy of Sciences (IF PAN), al. Lotnikow 32/46, 02-668 Warsaw, Poland
Biomacromolecules, 2008, 9 (7), pp 2087–2090
DOI: 10.1021/bm800421s
Publication Date (Web): June 25, 2008
Copyright © 2008 American Chemical Society
* To whom correspondence should be addressed. E-mail: tomasz.kowalczyk@ippt.gov.pl.
,
†

Institute of Fundamental Technological Research.

,
‡

Institute of Physics.

Abstract

Abstract Image

Electrospinning of the globular protein, bovine serum albumin (BSA), was optimized to obtain proteinous fibers suitable as biosensors. It was shown that the as-spun protein preserves its native form, whereas solubility of the cross-linked in the ambient conditions BSA nanofibers evidently decreases. Insoluble BSA fibers can be easily modified to be used as two-dimensional biosensors. Here, we show the micro pH sensor obtained from the BSA fiber stained with a fluorescein derivative (FITC).

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History

  • Published In Issue July 14, 2008
  • Article ASAPJune 25, 2008
  • Received: April 17, 2008
    Revised: May 06, 2008

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