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Biological Fingerprinting Analysis by Liquid Chromatography/Mass Spectrometry for Evaluation of DNA Structural Selectivity of Multiple Compounds in Natural Products

Xingye Su,^† Liang Kong,^† Xin Li,^† Xueguo Chen,^† Ming Guo,^‡ and Hanfa Zou*^†

National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China, and Department of Chemistry and Chemical Technology, Dalian University, Dalian 116622, China

J. Comb. Chem., 2006, 8 (4), pp 544–550

DOI: 10.1021/cc060039l

Publication Date (Web): June 9, 2006

†

National Chromatographic R. & A. Centre.

‡

Department of Chemistry and Chemical Technology.

To whom correspondence should be addressed. Phone: +86-411-84379610. Fax: +86-411-84379620. E-mail: hanfazou@dicp.ac.cn.

Abstract

A method by combination of centrifugal ultrafiltration (CUF) sampling with liquid chromatography−mass spectrometry (LC−MS) analysis was established to evaluate the DNA structure and sequence selectivity of the multiple compounds in a small molecule library. The developed method was applied to analyze the extracts of natural products Coptis chinensis Franch and Rheum palmatum (L.). From the obtained biological fingerprinting chromatograms, 7 compounds in C. chinensis Franch and 11 in R. palmatum (L.) were screened out as DNA binding agents. Most of these compounds were identified by standards and LC−MS analysis after the sample pretreatment with the DNA immobilized cartridge. DNA structural binding preference of the multiple active compounds in these two extracts was then evaluated simultaneously without purification.

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