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An Integrated Approach to the Undergraduate Biochemistry Laboratory
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Abstract
Undergraduate biochemistry laboratories traditionally expose students to biochemical techniques through a series of independent and usually unrelated laboratory exercises. Efforts to reorganize and update the undergraduate biochemistry laboratory at Texas Tech University have centered upon the development of a series of laboratory experiments that focus on a single biological system, the complex 11 (succinate:ubiquinone oxidoreductase) of Escherichia coli. Students are provided a computer-aided research environment in which to gain practical training in molecular biology, protein purification and enzyme kinetics. The laboratory schedule includes exercises on the succinate dehydrogenase operon (sdh) DNA sequence, and experiments that deal with isolation and characterization of sdh operon DNA, extraction and purification of complex 11 and characterization of complex 11 subunit structure and kinetic parameters. This unified approach to the biochemistry teaching laboratory is specifically designed to impact undergraduate student preparation for future studies, providing exposure to fundamental techniques of biochemistry experimentation and simulating the focused, single system, environment of a research laboratory.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
BiochemistryKeywords (Subject):
Nucleic acids / DNA / RNACiting Articles
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This article has been cited by 5 ACS Journal articles (5 most recent appear below).
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- Received: August 03, 2009
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