Prev. Article Next Article Table of Contents

Lab-Expt

Rapid Microscale Isolation and Purification of Yeast Alcohol Dehydrogenase Using Cibacron Blue Affinity Chromatography

Citing Articles

Your current credentials do not allow retrieval of the full text.

Purchase the full-text

PDF/HTML,
figures/images,
references and tables,
(where available)

Chad Morgan and Neil Moir

J. Chem. Educ., 1996, 73 (11), p 1040

DOI: 10.1021/ed073p1040

Publication Date (Web): November 1, 1996

Section:

History, Education, and Documentation

Abstract

A rapid microscale procedure has been developed for the isolation and purification of yeast alcohol dehydrogenase. Glass beads are used for cytolysis, PEG precipitation for partial purification and a cibacron blue affinity column for the final step. A 27.5 fold purification can be achieved in 2 - 3 hours.

Keywords (Audience):

Upper-Division Undergraduate

Keywords (Domain):

Biochemistry

Keywords (Feature):

The Microscale Laboratory

Keywords (Pedagogy):

Hands-On Learning / Manipulatives

Keywords (Subject):

Microscale Lab

View: Hi-Res PDF | PDF w/ Links

Citing Articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 2 ACS Journal articles (2 most recent appear below).

Kinetics of Alcohol Dehydrogenase-Catalyzed Oxidation of Ethanol Followed by Visible Spectroscopy
Kestutis Bendinskas , Christopher DiJiacomo , Allison Krill and Ed Vitz
Journal of Chemical Education2005 82 (7), 1068
- Kinetics of Alcohol Dehydrogenase-Catalyzed Oxidation of Ethanol Followed by Visible Spectroscopy
  Kestutis Bendinskas , Christopher DiJiacomo , Allison Krill and Ed Vitz
  Journal of Chemical Education2005 82 (7), 1068
  A two-week biochemistry experiment was introduced in the second-semester general chemistry laboratory to study the oxidation of ethanol in vitro in the presence of the enzyme alcohol dehydrogenase (ADH). This reaction should pique student interest because ...
  Abstract | Hi-Res PDF | PDF w/ Links
A Project-Oriented Biochemistry Laboratory Course
Paul A. Craig
Journal of Chemical Education1999 76 (8), 1130
- A Project-Oriented Biochemistry Laboratory Course
  Paul A. Craig
  Journal of Chemical Education1999 76 (8), 1130
  A project-oriented laboratory course has been designed to introduce students to the study of biochemistry as it is practiced. The course is designed to be a capstone experience for students enrolled in a variety of majors at the Rochester Institute of ...
  Abstract | Hi-Res PDF | PDF w/ Links