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Isolation and Purification of Calmodulin from Bovine Brain: A Biochemistry Experiment
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Abstract
This article describes a procedure for the purification of an enzyme from a readily available animal tissue. It allows for classical methods of enzyme purification to be examined by students. These methods include homogenization, centrifugation, isoelectric precipitation, and affinity chromatography. The experimental procedure is straightforward and most equipment is available at even small colleges and universities. Most teaching methods of protein isolation do not include an affinity chromatography step and the experiment benefits from the inclusion of this technique into the protocol.
Keywords (Audience):
Second-Year UndergraduateKeywords (Domain):
BiochemistryKeywords (Pedagogy):
Hands-On Learning / ManipulativesKeywords (Subject):
Proteins / PeptidesCiting Articles
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This article has been cited by 1 ACS Journal articles (1 most recent appear below).
The Isolation of Rubber from Milkweed Leaves. An Introductory Organic Chemistry Lab
Lisa Volaric and John HagenJournal of Chemical Education2002 79 (1), 91The Isolation of Rubber from Milkweed Leaves. An Introductory Organic Chemistry Lab
Lisa Volaric and John HagenJournal of Chemical Education2002 79 (1), 91We present an introductory organic chemistry lab in which students isolate rubber from the leaves of milkweed plants (Asclepias syriaca). Students isolated rubber with a recovery of 2.4 ± 1.8% and 1.8 ± 0.7% for the microscale and macroscale procedures, ...
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History
- Received: August 03, 2009
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